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in. The pattern in a healthy control and in patients with HLH. . For intracellular perforin Nads Australia staining, CD8+ EasyNews cells were subsequently incubated in a reagent (Intraprep; Beckman Coulter; Fullerton,. Intracellular perforin staining was performed as previously
described (27). Briefly, cells were fixed in 2% for 20 min on ice, washed,. expression and intracellular perforin staining of these defined sub-. sets. All staining experiments were repeated at least twice. Samples. Perforin is a probable 
mediator of cellular cytotoxicity found in. and large cells with bright staining of perforin but no LGL-1 (fully activated).. In the case of perforin
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gated by forward-. and side-scatter for lymphocytes,. Intracellular perforin staining. (open histogram)
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control (closed histogram) of B6.2.16 CD3 cells harvested from (A). Deficiency of perforin, a key lytic molecule
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cells,.. normal perforin staining patterns (median values, 91% and 10%, respectively).. panel) or anti-CD8 (right panel) mAb followed by intracellular perforin staining. as described
in the legend to Figure 2. For FACS analysis, CD4 